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DROSOPHILA NETWORK RESOURCES
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COMPARATIVE ANALYSES AND GENOME DATABASES
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- Berkeley Drosophila Genome Project (BDGP):
»BDGP, University of California, Berkeley, USA
- Drosophila Heterochromatin Genome Project (DHGP):
»DHGP, Lawrence Berkeley National Laboratory, USA
- D.melanogaster UCSC Genome Browser Gateway:
»University of California, Santa Cruz, USA
- FlyMine, an integrated database for Drosophila & Anopheles genomics:
»University of Cambridge, United Kingdom
- euGenes, Genomic Information for Eukaryotic Organisms,including Drosophila:
» euGenes, Indiana University, USA
- CluSTr protein sequence similarity analysis of Drosophila:
»CluSTr proteome analysis, EBI, UK
- Homophila, Human Disease to Drosophila Gene Database:
» Homophila, San Diego, USA
- InterPro protein domain analysis of Drosophila:
»InterPro proteome analysis, EBI, UK
- The Drosophila Transcription Factor Database:
»FlyTF.org, MRC Cambridge, UK
- Sebida, Sex Bias Database:
»Sebida, MPI Martinsried, Germany
- The McGill Drosophila melanogaster Genome Project:
»McGill Drosophila Genome Project, Canada
- Proteome analysis of Drosophila:
»Proteome analysis, EBI, UK
- Panther protein classification system:
»Panther Protein Classification System, Applied Biosystems & Celera, USA
- Drosophila melanogaster Exon Database (DEDB):
»DEDB, National University of Singapore
- TaxoDros, the database on Taxonomy of Drosophilidae:
»TaxoDros, University of Zurich, Switzerland
- Drosophilidae Taxonomic Database:
»Drosophilidae Taxonomic Database, Japan
- Drosophilidae Taxonomy - Collection of Papers:
»Japan Drosophila Database (JDD) on Taxonomy, Japan
- miRNA - Target Gene Prediction:
»miRNA - Target Prediction, EMBL, Heidelberg, Germany
- Mitotic Spindle Assembly Database:
»Genes Required for Mitotic Spindle Assembly in S2 cells, University of California, San Francisco, USA
- DNase I Footprint Database:
»University of Manchester Bioinformatics Resources, University of Manchester, UK
- Drosophila Polymorphism Database:
»Drosophila Polymorphism Database, Univ. Autònoma de Barcelona, Spain
- BDGP Single Nucleotide Polymorphism (SNP) Project:
»Berkeley Drosophila Genome Project, University of California, Berkeley, USA
- FlySNP Project:
»FlySNP, Austrian Academy of Sciences, Austria
- Drosophila Population Genomic Project (DPGP):
»DPGP, University of California, Davis, USA
- Heidelberg FlyArray Expression Project:
»Heidelberg FlyArray, ZMBH, Heidelberg, Germany
- FLIGHT, Fly database for the Integration of Genomic and High-Throughput data:
»FLIGHT Database, Ludwig Institute for Cancer Research, London, UK
- NHGRI Drosophila Genome Sequencing Proposals, Status of Sequencing:
»National Human Genome Research Institute, Bethesda, MD, USA
- Assembly/Alignment/Annotation of 12 related Drosophila species:
»Assembly/Alignment/Annotation, LBNL, USA
- Whole genome comparative analysis of D. melanogaster and D. pseudoobscura:
»Whole genome analysis, LBNL, USA
- D. pseudoobscura genome project:
»Human Genome Sequencing Center, Baylor College of Medicine, USA
- Drosophila simulans and yakuba sequencing projects:
»DPGP, University of California, Davis, USA
- D. simulans genome project:
»Genome
Sequencing Center, Washington University, USA
- D. yakuba genome project:
»Genome Sequencing Center, Washington University, USA
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SEQUENCE TRACES OF DROSOPHILA GENOMES
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- D. ananassae genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. erecta genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. grimshawi genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. melanogaster genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. mojavensis genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. persimilis genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. pseudoobscura genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. sechellia genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. simulans genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. virilis genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. willistoni genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
- D. yakuba genomic sequence trace archives:
»NCBI Trace Archives, USA
»Ensembl FTP Trace Repository, EBI, UK
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GENETIC AND PROTEIN INTERACTION DATABASES
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- BioGRID, The General Repository for Interaction Datasets
»BioGRID, Mount Sinai Hospital, Toronto, Canada
- CuraGen Drosophila Interaction Database
»Drosophila Interaction Database, CuraGen, USA
- Drosophila PIMRider, Drosophila Protein Interaction Map
»Drosophila PIMRider, Hybrigenics, Paris, France
- Flynet, Drosophila Protein-protein Interactions
»Flynet, Johns Hopkins University, Baltimore, MD, USA
- IM Browser, Drosophila Interactions Database
»IM Browser, Wayne State University, Detroit, MI, USA
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SEQUENCE ANALYSIS
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- Gene-Finder for Drosophila:
»Gene Finding, FGENESH, SoftBerry
- GenePalette, a tool for genome sequence visualization and navigation:
»GenePalette, University of California, San Diego, USA
- Genie Gene finder for Drosophila:
»Genie, USA
- GRAIL Gene Finder for Drosophila:
»GRAIL, USA
- McPromoter Promoter Prediction for Drosophila:
»McPromoter, MIT, USA
- Neural Network Promoter Prediction for Drosophila:
»Neural Network Promoter Prediction, USA
- REDfly, Regulatory Element Database for Drosophila:
»REDfly, University at Buffalo, New York, USA
- cis-analyst, to search the Drosophila genome for clusters of binding sites:
»cis-analyst, USA
- Target Explorer, to identify clusters of transcription factor binding sites:
»Target Explorer, Columbia University, New York, USA
- Stubb site, to find clusters of cis-regulatory binding sites:
»Stubb web site, Rockefeller University, New York, USA
- PREdictor, to identify Polycomb/Trithorax response elements:
»PREdictor, Bielefeld University, Germany
- Splice Site Prediction for Drosophila:
»Splice Site Prediction, USA
- TransTerm, a Translational Signal Database:
»TransTerm, Dunedin, New Zealand
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ATLASES AND IMAGES
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- CSIRO Entomology Anatomical Atlas of Flies:
»CSIRO Entomology, Australia
- Electron micrograph maps of Drosophila melanogaster polytene chromosomes:
»Sorsa Maps, Helsinki, Finland
- FlyBrain, an Online Atlas and Database of the Drosophila Nervous System:
»FlyBrain, University of Freiburg, Freiburg, Germany
»FlyBrain, University of Arizona, Tucson, USA
»FlyBrain, NIBB, Okazaki, Japan
- FlyEx, a Database of Segmentation Gene Expression in Drosophila:
»FlyEx, St. Petersburg State Polytechnical University, Russia
»FlyEx, Stony Brook University, USA
- FlyExpress, an Expression Pattern Search Engine:
»FlyExpress, Arizona State University, USA
- Fly Motor Axon Page:
»Motor Axon, California Institute of Technology, USA
- FlyTrap, a GFP Protein Trap Database:
»FlyTrap, Yale University, USA
- flytrap, a database of P{GAL4} enhancer traps and their expression in brains:
»flytrap, University of Edinburgh, UK
- FlyView, a Drosophila Image Database:
»FlyView, Muenster, Germany
- FlyMove, an Image, Movie and Interactive Shockwave Resource:
»FlyMove, Muenster, Germany
- GETDB, a Gal4 enhancer trap database:
»GETDB, National Institute of Basic Biology, Okazaki, Japan
- Patterns of gene expression in Drosophila embryogenesis:
»Gene expression database, BDGP, USA
- Protein trap database, a GFP embryo expression database:
»Protein trap database, Villefranche-sur-mer, France
- Scans of Bridges' original 1935 polytene drawings for D. melanogaster:
»GEM Website, University of Hawaii, USA
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MISCELLANEOUS
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- Drosophila Information Service:
»DIS, Norman, Oklahoma, USA
- FlyNome, a database of Drosophila nomenclature:
»FlyNome, USA
- FlyPNS, embryonic and larval peripheral nervous system:
»FlyPNS, USA
- Jfly , a data depository for the fly and other insects, with an emphasis for the Japanese-speaking fly community:
»Jfly, University of Tokyo, Japan
- The WWW Virtual Library--Drosophila:
»Drosophila Virtual Library
- Tracheal Database, information on tracheal development in Drosophila:
»Tracheal Database, Switzerland
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DROSOPHILA MATERIAL RESOURCES
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GENOMIC CLONES
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BAC GENOMIC CLONES AND FILTERS
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Only one BAC clone library is now available, made from DNA of the
same y1 ; cn1 bw1 sp1
stock as was used for the Berkeley Drosophila Genome Project P1 clones.
This BACR library was made for the BDGP by
K. Osoegawa, A. Mammoser,
and P. de Jong.
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BACR clones (18,432 clones in pBACe3.6; average clone size is
160-Kb) are available from BACPAC Resources at:
http://bacpac.chori.org/dromel98.htm
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P1 GENOMIC CLONES
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As the P1 library is largely obsolete, the BDGP is discouraging
the use of P1 clones. If there is a compelling scientific reason that a
corresponding BAC is not sufficient, requests for P1 clones can be
directed to the BDGP.
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For additional information about the Berkeley Drosophila Genome
Project see http://www.fruitfly.org.
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COSMID GENOMIC CLONES AND FILTERS
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EDGP cosmids are available from the Geneservice:
http://www.geneservice.co.uk/products/clones/drosophila.jsp.
Cosmids are available either singly or on high-density filters.
The members of the European Drosophila Genome mapping
consortium are:
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F.C. Kafatos, C. Louis, C. Savakis, I. Siden-Kiamos.
Institute
of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete.
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D.M. Glover and R.D.C. Saunders,
Department of Biochemistry,
University of Dundee, Dundee, Scotland.
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M. Ashburner,
Department of Genetics, University of Cambridge,
Cambridge, England.
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J. Modolell,
Centro de Biologia Molecular, Universidad
Autonoma, Madrid, Spain.
Supported by the Stimulation Action Programme of the European
Community, with initial funding from the Fondation Schlumberger.
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YAC GENOMIC CLONES
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SIBLING SPECIES GENOMIC LIBRARIES
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cDNA (EST) CLONES AND LIBRARIES
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ARRAYS AND PRIMERS
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International Drosophila Array Consortium (INDAC)
An international consortium to collaborate in the development
and aquisition of microarray resources for the benefit of the entire
research community. More information is available at http://www.indac.net/
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Affymetrix
The Affymetrix GeneChip Drosophila Genome Array based on Release
3.1 annotations can be obtained at http://www.affymetrix.com/products/arrays/specific/fly_2.affx
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Eurogentec
The Eurogentec Ready-to-spot PCR sets for MicroArrays based on
Release 2 annotations, DGC Release 1.0, and Sanger Center annotations
can be obtained at http://www.eurogentec.be/code/en/page_02.asp?Page=377
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FlyChip
FlyChip is a publicly funded project to provide a non-profit
microarray resource for the UK Drosophila research community. More
information is available at http://www.flychip.org.uk/
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Drosophila Genomics Resource Center (DGRC)
Produces and distributes DNA microarray slides. More information
is available at http://dgrc.cgb.indiana.edu/
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UK Drosophila Affymetrix Microarray Service
A publicly funded microarray service for the UK Drosophila
research community. More information is available at
http://www.mblab.gla.ac.uk/igf/
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STOCKS
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BDGP/Baylor Gene Disruption Project
Information about ordering P{GT1}(BG), P{SuporP}(KG), and
P{Epgy2}(EY) lines from Baylor College of Medicine, Texas, USA can be
found at http://flypush.imgen.bcm.tmc.edu/pscreen/
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Bloomington Stock Center
A large collection of stocks at Indiana Univerisity, USA can be
obtained at http://flystocks.bio.indiana.edu
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DrosDel Project
Information about ordering isogenic deficiency lines at the
University of Cambridge, UK can be found at http://www.drosdel.org.uk/
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Drosophila Gene Search Project
Information about ordering P{GS} lines can be found at http://gsdb.biol.metro-u.ac.jp/~dclust/index.html
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Ehime Drosophila Species Stock Center of Japan
A collection of wild-type strains of D. melanogaster and strains
of some 30 species can be found at http://kyotofly.kit.ac.jp/ehime/
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Exelixis Collection at Harvard Medical School
Information about ordering the Exelixis stocks at Harvard Medical
School can be found at http://drosophila.med.harvard.edu/
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FlyTrap Stock Collection
GFP protein trap lines from the FlyTrap project can be obtained at
Yale University, USA at http://flytrap.med.yale.edu/
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FlyView Stock Collection
Enhancer trap lines from the FlyView project can be obtained at
University of Muenster, Germany at http://flyview.uni-muenster.de/
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GETDB--Gal4 Enhancer Trap Insertion Database
Information about ordering Gal4 enhancer trap lines generated and
analyzed by NP Consortium from the Drosophila
Genetic Resource Center
in Kyoto, Japan can be found at http://flymap.lab.nig.ac.jp/~dclust/getdb.html
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Japanese Laboratories
Stock lists from individual laboratories in Japan are available at
http://www.grs.nig.ac.jp/fly/CENTER.e.html
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Kyoto Stock Center
Information about ordering P element insertion lines,
deficiency, or duplication kits at the Kyoto Institute of Technology,
Japan can be found at http://dbs.kit.ac.jp/stocks/
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NIG-FLY: Fly Stocks of the National Institute of Genetics
Stocks for RNA interference experiments may be obtained from the NIG RNAi fly unit at http://www.shigen.nig.ac.jp/fly/nigfly/
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Szeged Stock Centre
Information about ordering P{lacW}, P{EP}, P{RS3}, and P{RS5}
insertion lines at the University of Szeged, Hungary can be obtained at
http://expbio.bio.u-szeged.hu/fly/index.php
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Tucson Stock Center
Approximately 270 different Drosophila species at University of
Arizona, USA can be obtained at http://stockcenter.arl.arizona.edu
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Vienna Drosophila RNAi Center
Stocks for RNA interference experiments can be obtained from the IMP, Austria at
http://stockcenter.vdrc.at/control/main
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EXPRESSION VECTORS
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Drosophila Gateway Vectors
A set of 68 Gateway-based vectors designed to express
epitope-tagged proteins in Drosophila culture cells or flies, available
at the DGRC
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CELL LINES AND HYBRIDOMAS
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ANTIBODIES
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RNA
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RNAi
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TRANSGENIC, TARGETED MUTATION, AND OTHER SERVICES
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Aktogen behaviour genetic services
Aktogen at Cambridge University offers a wide range of services to the
fruit fly community such as behaviour tests, behaviour and other
equipment, custom made genetic constructs and transgenic services. http://www.aktogen.com/
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BestGene Transgenic Service
The BestGene Drosophila Embryo Injection service provides
partial to full service ranging from DNA preparation, embryo
microinjection to balancing crosses. More information is available at http://www.thebestgene.com/
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Drosophila TILLING Project
The goal of Fly-TILL is to rapidly deliver to the Drosophila
community EMS-induced mutations in requested genes via high-throughput
reverse genetic screens. More information is available at http://tilling.fhcrc.org:9366/fly/
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Duke University Non-Mammalian Model Systems Flyshop
The Duke University Non-Mammalian Model Systems Flyshop provides
P-element transformation, dsRNA injection, embryo sorting, and inverse
PCR services. More information is available at http://www.biology.duke.edu/model-system
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Genetic Services
Genetic Services provides the Drosophila community with services
including transgenic production, injection work, genetic screening,
stock creation, construct production and consulting. More information
is available at http://www.geneticservices.com
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Genetivision, Inc.
Genetivision offers a transgenic fly service for both
delta 2-3 transposase-mediated P-element transgenesis and Phi-C31
mediated site-specific integration. More information
is available at http://www.genetivision.com/
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Model Biosystems Drosophila Stock Center
The stock center in Bangalore offers a stock maintenance service at a very reasonable price to the Drosophila researchers all over the world
http://www.modelbiosystems.com
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Rainbow Transgenic Flies
Rainbow Transgenic Flies provides professional microinjection
services to the fly community. More information is available at http://www.rainbowgene.com/
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Transgenic Drosophila Fly Core
The Cutaneous Biology Research Center (CRBC) Fly Core at
Massachusetts General Hospital/Harvard Medical School provides
microinjection services to create transgenic Drosophila fly strains.
More information is available at http://www.flycore.com
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Union Biometrica
COPAS Select flow cytometry instruments are able to analyze and
dispense Drosophila embryos on the basis of size and fluorescent signals. More
information is available at http://www.unionbio.com/
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VANEDIS Drosophila injection service
The VANEDIS Drosophila injection service injects embryos with your
construct of interest. More information is available at http://www.vanedis.no
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